RT-PCR artifacts from processed pseudogenes.
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چکیده
منابع مشابه
RT-PCR artifacts from processed pseudogenes.
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متن کاملPitfalls of processed pseudogenes in RT-PCR.
Pseudogenes are genes with high sequence homology to functional genes that have been rendered obsolete by mutations, preventing their expression. Several genes, such as αand β-globin, have been shown to have duplicated sequences representing nonfunctional genes or pseudogenes. The presence of pseudogenes is common; not every DNA duplication produces a functional gene. Cyclophilin (CyP), an 18-k...
متن کاملDesign and testing of beta-actin primers for RT-PCR that do not co-amplify processed pseudogenes.
Quantitative reverse transcription polymerase chain reaction (RT-PCR) is being used increasingly as an alternative to Northern blots analysis or RNase protection assays for quantitation of gene expression. To quantify different samples, measurements are often normalized using the expression of so-called "housekeeping" genes, such as cytoplasmic beta-actin or glyceraldehyde-3-phosphate dehydroge...
متن کاملExclusive amplification of cDNA template (EXACT) RT-PCR to avoid amplifying contaminating genomic pseudogenes.
Genomic DNA contamination within RNA samples has important implications for RT-PCR, particularly if there is a pseudogene related to the gene under investigation, because amplification from pseudogenes and reverse-transcribed cDNA can be very difficult to distinguish. Methods to remove DNA contamination cannot guarantee the absolute absence of DNA from the sample without a loss of RNA quantity ...
متن کاملProcessed pseudogenes acquired somatically during cancer development
Cancer evolves by mutation, with somatic reactivation of retrotransposons being one such mutational process. Germline retrotransposition can cause processed pseudogenes, but whether this occurs somatically has not been evaluated. Here we screen sequencing data from 660 cancer samples for somatically acquired pseudogenes. We find 42 events in 17 samples, especially non-small cell lung cancer (5/...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1991
ISSN: 1088-9051
DOI: 10.1101/gr.1.1.70